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KMID : 1098420050130040138
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Korean Journal of Medicinal Crop Science
2005 Volume.13 No. 4 p.138 ~ p.140
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In vitro Growth of Shoot Derived from Shoot Tip in Asparagus cochinchinensis (Lour.) Merr
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Choo Byung-kil
Kim Dae-Hyang
Jung Ju-Ri
Lim Ju-Rak
Park Chun-Bong
Ko Byoung-Seob
Ryu Jeon-Ho
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Abstract
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This study was carried out to investigate the optimal conditions for efficient in vitro growth of Asparagus cochinchinensis (Lour.) Merr. shoot derived from shoot tip. Shoots were successfully cultured in MS medium. It was found that 23^{circ}C{sim}25^{circ}C were suitable for shoot growth. The growth of shoot was greatly influenced by cytokinins. The shoots derived from shoot tip were well elongated on MS medium supplemented with BA and zeatin. In vitro shoots were very poor or no growing. Especially, 3.0 mg/l BA on MS medium was very effective in elongation of shoot. Root formation from in vitro growth of plant let was achieved on MS medium supplemented with 0.5 mg/l IBA. The results suggest that selection of plant growth regulator could be important factor to achieve an efficient in vitro growth.
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KEYWORD
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Asparagus cochinchinensis (Lour.) Merr., shoot growth, Root formation
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